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KMID : 0880220190570020101
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Journal of Microbiology
2019 Volume.57 No. 2 p.101 ~ p.106
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Blue-Red LED wavelength shifting strategy for enhancing beta-carotene production from halotolerant microalga, Dunaliella salina
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Han Sang-Il
Kim Sok
Lee Chang-Su
Choi Yoon-E
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Abstract
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In the present study, to improve the photosynthetic betacarotene productivity of Dunaliella salina, a blue-red LED wavelength-shifting system (B-R system) was investigated. Dunaliella salina under the B-R system showed enhanced density and beta-carotene productivity compared to D. salina cultivated under single light-emitting diode light wavelengths (blue, white, and red light-emitting diode). Additionally, we developed blue light-adapted D. salina (ALE-D. salina) using an adaptive laboratory evolution (ALE) approach. In combination with the B-R system applied to ALE-D. salina (ALE B-R system), the beta-carotene concentration (33.94 ± 0.52 μM) was enhanced by 19.7% compared to that observed for the non-ALE-treated wild-type of D. salina (intact D. salina) under the B-R system (28.34 ± 0.24 μM).
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KEYWORD
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Dunaliella salina, beta-carotene, light-emitting diode, wavelength shift, adaptive laboratory evolution
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